Luminescent bacteria and didactics
Luminescent bacteria (particularly the organism Photobacterium phosphoreum) are well-suited for use in schools. These are some features which make Photobacterium phosphoreum the good organism to didactical usage.:
If you are not
able to get luminous bacteria from laboratory cultures you can
isolate it from sea-fish or squid
Here you can find detailed description how to do it ...
|Isolation of pure
cultures of bacteria - Vibrio
materials from Indiana Biolab written by Harold Eddleman
You do not need many ingredients and time to prepare microbiological medium for cultivation of Photobacterium phosphoreum.
|MICROBIOLOGICAL MEDIA FOR CULTIVATION OF LUMINESCENT BACTERIA|
The room temperature (20 - 22°C) is the optimal for growth of luminous bacteria. So, you do not need special equipment (e.g. incubators) to cultivate these bacteria.
Sometimes I observed weak luminescence of growing bacteria after a few hours of incubation but well visible, bright glowing colonies of Photobacterum phosphoreum you can observe after 24 - 48 h of incubation.
The bacterial bioluminescence is one from the most spectacular phenomena which can be observed in microbiological courses. This can diversify the classes and develop interest of young people.
After all taking care is necessary like in case of work with any other microorganisms.
luminescence causes that on the constant, microbiological medium
the brief appreciation of purity of isolation is possible - even
if processed through beginners.
Since bioluminescence is an unusual trait, it is very useful for teaching molecular biology methods such as isolation of DNA, making a gene library, cloning of gene of luciferase, transformation with plasmid carrying luciferase gene, luciferase gene amplification (PCR) etc.
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